Anti- Inosine triphosphate pyrophosphatase 抗体 (ab79967)

製品の概要

  • 製品名Anti- Inosine triphosphate pyrophosphatase antibody
    Inosine triphosphate pyrophosphatase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Inosine triphosphate pyrophosphatase
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Inosine triphosphate pyrophosphatase.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in HepG2 Whole Cell Lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab79967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
WB
  • 追加情報ICC/IF: Use at a concentration of 5 µg/ml.
    WB: Use at an assay dependent dilution. Detects a band of approximately 24 kDa (predicted molecular weight: 21 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Hydrolyzes ITP and dITP to their respective monophosphate derivatives. Xanthosine 5'-triphosphate (XTP) is also a potential substrate. May be the major enzyme responsible for regulating ITP concentration in cells.
    • 組織特異性Ubiquitous. Highly expressed in heart, liver, sex glands, thyroid and adrenal gland.
    • 関連疾患Defects in ITPA are the cause of inosine triphosphate pyrophosphohydrolase deficiency (ITPA deficiency) [MIM:147520]. It is a common inherited trait characterized by the abnormal accumulation of inosine triphosphate (ITP) in erythrocytes and also leukocytes and fibroblasts. The pathological consequences of ITPA deficiency, if any, are unknown. However, it might have pharmacogenomic implications and be related to increased drug toxicity of purine analog drugs. Three different human populations have been reported with respect to their ITPase activity: high, mean (25% of high) and low activity. The variant Thr-32 is associated with complete loss of enzyme activity, may be by altering the local secondary structure of the protein. Heterozygotes for this polymorphism have 22.5% of the control activity: this is consistent with a dimeric structure of the enzyme.
    • 配列類似性Belongs to the HAM1 NTPase family.
    • 細胞内局在Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • C20orf37 antibody
      • dJ794I6.3 antibody
      • HLC14-06-P antibody
      • Inosine triphosphatase (nucleoside triphosphate pyrophosphatase) antibody
      • Inosine triphosphatase antibody
      • inosine triphosphatase-A antibody
      • Inosine triphosphate pyrophosphatase antibody
      • Inosine triphosphate pyrophosphohydrolase antibody
      • Itpa antibody
      • ITPA_HUMAN antibody
      • ITPase antibody
      • My049 antibody
      • My049 protein antibody
      • Non canonical purine NTP pyrophosphatase antibody
      • Non standard purine NTP pyrophosphatase antibody
      • NTPase antibody
      • nucleoside triphosphate diphosphatase antibody
      • Nucleoside triphosphate pyrophosphatase antibody
      • OK/SW-cl.9 antibody
      • OTTHUMP00000030094 antibody
      • OTTHUMP00000160459 antibody
      • Putative oncogene protein hlc14-06-p antibody
      see all

    Anti- Inosine triphosphate pyrophosphatase antibody 画像

    • Anti- Inosine triphosphate pyrophosphatase antibody (ab79967) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 21 kDa
      Observed band size : 24 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 125 kDa,70 kDa,75 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 10 minutes
    • ICC/IF image of ab79967 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79967, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 5µg/ml.

    Anti- Inosine triphosphate pyrophosphatase antibody (ab79967) 使用論文

    ab79967 has not yet been referenced specifically in any publications.

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