製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to C21orf66
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide from within a region of N terminal amino acids 215-264 (PGEIPDAAFI HAARKKRQMA RELGDFTPHD NEPGKGRLVR EDENDASDDE) of Human C21orf66 (NP_037461)

  • ポジティブ・コントロール
    • Fetal muscle lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab82669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 105 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Possible transcription factor.
  • 組織特異性
    Ubiquitous.
  • 配列類似性
    Belongs to the GCF family.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BM 020 antibody
    • BM020 antibody
    • Chromosome 21 open reading frame 66 antibody
    • FSAP105 antibody
    • Functional spliceosome-associated protein 105 antibody
    • GC rich sequence DNA binding factor candidate antibody
    • GC-rich sequence DNA-binding factor 1 antibody
    • GCFC antibody
    • GCFC1 antibody
    • GCFC1_HUMAN antibody
    • PAX3 and PAX7 binding protein 1 antibody
    • PAXBP1 antibody
    see all

画像

  • Anti- C21orf66 antibody (ab82669) at 1 µg/ml (5% skim milk/ PBS buffer) + fetal muscle cell lysate at 10 µg with gel concentration: 6-18%

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 105 kDa
    Observed band size : 105 kDa

プロトコール

参考文献

ab82669 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunocytochemistry
Sample
Mouse Cell (Muscle myoblast)
Permeabilization
Yes - 0.5% PBST
Specification
Muscle myoblast
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Paraformaldehyde
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投稿 May 09 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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